This function calculates TPM values from raw expression counts and gene lengths.
TPM is a normalization method that accounts for both sequencing depth and gene length,
allowing comparison of gene expression levels within and between samples.
Usage
estimateTPM(exp, gene_lengths)
Arguments
- exp
A numeric matrix or data frame of raw expression counts. Rows represent genes, columns represent samples.
- gene_lengths
A numeric vector of gene lengths (in kilobases or base pairs) corresponding to the rows of exp.
Must be the same length as the number of rows in exp.
Value
A numeric matrix of TPM-normalized expression values with the same dimensions as exp.