Normalize Gene Expression Data

This function normalizes gene expression counts in a `SummarizedExperiment` object using either `rlog` or `vst` normalization, depending on the number of samples.

Usage

normalize_gexp(exp_data)

Arguments

exp_data

A `SummarizedExperiment` object containing the gene expression data with a `counts` assay.

Value

A `SummarizedExperiment` object with an additional `norm` assay containing the normalized expression data.

Details

The function applies one of two normalization methods: - `rlog` (regularized log transformation) is used for datasets with fewer than 30 samples. - `vst` (variance-stabilizing transformation) is used for datasets with 30 or more samples.

The normalized data is stored in a new assay named `"norm"`.